Fig. 4. Concentrations of pro-inflammatory (IL-6 and TNF-α) and pro-angiogenic (MCP-1 and VEGF) cytokines produced by 4T1 breast cancer cells cultured in RAW 264.7 macrophage-conditioned media: non-treated (CM-NT) or PBP-treated (CM-PBP). Cells were cultivated in 50% conditioned media for 72 h, and then the levels of cytokines in the supernatants were measured by ELISA methods. Concentrations of cytokines in original RAW 264.7 macrophage-conditioned media (CM-NT only and CM-PBP only), supernatants collected after cell stimulation with only PBP (PBP) or incubation of cells in control medium (vehicle) for 72 h was also assessed. Data are shown as the mean ± SEM of six independent experiments. Asterisks show significant differences between the vehicle and all other tested supernatants (***P<0.001; **P<0.01, *P<0.05). Hash marks indicate significant differences between CM-NT and CM-PBP or CM-NT only and CM-PBP only, respectively (^{# # #}P<0,001; ^#P<0.05).